THE CHROMATOGRAPHY OF PHOTOSYNTHETIC PIGMENTS IN YOUR research laboratory notebook: 1) Go to The biological science Place on termination and critique this lab. Put any notes you want in your lab notebook. DO NOT SIMPLY PRINT THE LAB AND GLUE IT INTO YOUR NOTEBOOK!! 2) I cut the food coloured bands fall off of the chromatograms you prepared. thus I placed the bands of separately type in a few ml of acetone. This gave me four small establish tubes, distributively containing an acetone solution of one of the pigments. Students then mensural the absorbance of each pigment at wavelengths from 400nm to 700nm. 3) Construct a line map of the absorbance data. Wavelength is the independent variable. Add a descriptive title. The graph should hurt four curves. 4) Consult the graph in your text. If you can, subprogram it to call and label the curves on your own graph. 5) What is the difference amongst an absorption spectrum and an troops ac tion spectrum? 6) Diagram and label a system that would have you to measure a plants action spectrum. 7) What color is DPIP when reduced? When oxidized?
8) In intact chloroplasts where do electrons go when they are ejected by chlorophyl? 9) If you illuminate a ripple of mashed chloroplasts and DPIP, is the DPIP oxidized or reduced? 10) As the execute depict in #9 proceeds, how does the color change? What should fall out to the mixtures absorbance? 11) During chromatography the solvent front moves 12 cm while a band moves 7 cm. Calculate the Rf value. 12) Explain the function of each: a) chlorophyl a b) chloroph! yll b c) carotenoidsIf you want to get a full essay, regularise it on our website:
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